John A. Kiernan
University of Western Ontario
Publications 139
#1P. Joy Dunmore-Buyze (UWO: University of Western Ontario)H-Index: 2
#2Charmainne Cruje (UWO: University of Western Ontario)H-Index: 1
Last.Maria Drangova (UWO: University of Western Ontario)H-Index: 35
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Virtual histology – utilizing high-resolution three-dimensional imaging – is becoming readily available. Micro-computed tomography (micro-CT) is widely available and is often coupled with x-ray attenuating histological stains that mark specific tissue components for 3D virtual histology. In this study we describe a new tri-element x-ray attenuating stain and perfusion protocol that provides micro-CT contrast of the entire vasculature of an intact mouse. The stain – derived from an established hi...
#1John A. Kiernan (UWO: University of Western Ontario)H-Index: 36
AbstractPrevious investigators have disagreed about whether hemalum stains DNA or its associated nucleoproteins. I review here the literature and describe new experiments in an attempt to resolve the controversy. Hemalum solutions, which contain aluminum ions and hematein, are routinely used to stain nuclei. A solution containing 16 Al3+ ions for each hematein molecule, at pH 2.0–2.5, provides selective progressive staining of chromatin without cytoplasmic or extracellular “background color.” Su...
#1Víctor Carriel (UGR: University of Granada)H-Index: 17
#2Fernando Campos (UGR: University of Granada)H-Index: 7
Last.John A. Kiernan (UWO: University of Western Ontario)H-Index: 36
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Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and proces...
#1John A. KiernanH-Index: 36
Barr's the human nervous system , Barr's the human nervous system , کتابخانه دیجیتال جندی شاپور اهواز
#1John A. Kiernan (UWO: University of Western Ontario)H-Index: 36
Only primates have temporal lobes, which are largest in man, accommodating 17% of the cerebral cortex and including areas with auditory, olfactory, vestibular, visual and linguistic functions. The hippocampal formation, on the medial side of the lobe, includes the parahippocampal gyrus, subiculum, hippocampus, dentate gyrus, and associated white matter, notably the fimbria, whose fibres continue into the fornix. The hippocampus is an inrolled gyrus that bulges into the temporal horn of the later...