Bob Cudney
Protein crystallizationCrystallizationMacromoleculeCrystalCrystallography
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Publications 4
© 2014 International Union of Crystallography. For the successful X-ray structure determination of macromolecules, it is first necessary to identify, usually by matrix screening, conditions that yield some sort of crystals. Initial crystals are frequently microcrystals or clusters, and often have unfavorable morphologies or yield poor diffraction intensities. It is therefore generally necessary to improve upon these initial conditions in order to obtain better crystals of sufficient quality for ...
33 CitationsSource
#1Alexander McPherson (UC: University of California)H-Index: 51
#2Chieniang NguyenH-Index: 1
Last. Bob CudneyH-Index: 4
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We are developing an alternate strategy for the crystallization of macromolecules that does not, like current methods, depend on the optimization of traditional variables such as pH and precipitant concentration, but is based on the hypothesis that many conventional small molecules might establish stabilizing, intermolecular, non covalent crosslinks in crystals, and thereby promote lattice formation. To test the hypothesis, we carried out preliminary experiments encompassing 18,240 crystallizati...
4 CitationsSource
#1Robert ViolaH-Index: 3
#2Peter CarmanH-Index: 2
Last. Bernhard Rupp (UCI: University of California, Irvine)H-Index: 3
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High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughpu...
23 CitationsSource
#1Alexander McPherson (UCI: University of California, Irvine)H-Index: 51
#2Bob CudneyH-Index: 4
Abstract Based on a hypothesis that various small molecules might establish stabilizing, intermolecular, non covalent crosslinks in protein crystals and thereby promote lattice formation, we carried out three separate experiments. We assessed the impact of 200 chemicals on the propensity of 81 different proteins and viruses to crystallize. The experiments were comprised of 18 240 vapor diffusion trials. A salient feature of the experiments was that, aside from the inclusion of the reagent mixes,...
117 CitationsSource