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Stephen C. Kowalczykowski
University of California, Davis
244Publications
76H-index
18.8kCitations
Publications 238
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#1Shirin S. Jenkins (UC Davis: University of California, Davis)H-Index: 2
#2Steven K Gore (UC Davis: University of California, Davis)H-Index: 2
Last.Wolf Dietrich Heyer (UC Davis: University of California, Davis)H-Index: 48
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Saccharomyces cerevisiaeSrs2, in addition to its well-documented antirecombination activity, has been proposed to play a role in promoting synthesis-dependent strand annealing (SDSA). Here we report the identification and characterization of an SRS2 mutant with a single amino acid substitution (srs2-F891A) that specifically affects the Srs2 pro-SDSA function. This residue is located within the Srs2–Rad51 interaction domain and embedded within a protein sequence resembling a BRC repeat motif. The...
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#1James E. Graham (UC Davis: University of California, Davis)H-Index: 12
#2Kenneth J. Marians (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 39
Last.Stephen C. Kowalczykowski (UC Davis: University of California, Davis)H-Index: 76
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Summary It has been assumed that DNA synthesis by the leading- and lagging-strand polymerases in the replisome must be coordinated to avoid the formation of significant gaps in the nascent strands. Using real-time single-molecule analysis, we establish that leading- and lagging-strand DNA polymerases function independently within a single replisome. Although average rates of DNA synthesis on leading and lagging strands are similar, individual trajectories of both DNA polymerases display stochast...
43 CitationsSource
#1Yi Gong (Rockefeller University)H-Index: 3
#2Naofumi Handa (UC Davis: University of California, Davis)H-Index: 8
Last.Titia de Lange (Rockefeller University)H-Index: 80
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8 CitationsSource
2 CitationsSource
#1Jason C. Bell (University of California, Berkeley)H-Index: 7
The repair of DNA by homologous recombination is an essential, efficient, and high-fidelity process that mends DNA lesions formed during cellular metabolism; these lesions include double-stranded DNA breaks, daughter-strand gaps, and DNA cross-links. Genetic defects in the homologous recombination pathway undermine genomic integrity and cause the accumulation of gross chromosomal abnormalities—including rearrangements, deletions, and aneuploidy—that contribute to cancer formation. Recombination ...
26 CitationsSource
#1Jason C. Bell (UC Davis: University of California, Davis)H-Index: 7
#2Stephen C. Kowalczykowski (UC Davis: University of California, Davis)H-Index: 76
Homologous recombination maintains genomic integrity by repairing broken chromosomes. The broken chromosome is partially resected to produce single-stranded DNA (ssDNA) that is used to search for homologous double-stranded DNA (dsDNA). This homology driven ‘search and rescue' is catalyzed by a class of DNA strand exchange proteins that are defined in relation to Escherichia coli RecA, which forms a filament on ssDNA. Here, we review the regulation of RecA filament assembly and the mechanism by w...
55 CitationsSource
#1Juan S. Martinez (Curie Institute)H-Index: 1
#2Catharina von Nicolai (Curie Institute)H-Index: 3
Last.Aura Carreira (Curie Institute)H-Index: 11
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In somatic cells, BRCA2 is needed for RAD51-mediated homologous recombination. The meiosis-specific DNA strand exchange protein, DMC1, promotes the formation of DNA strand invasion products (joint molecules) between homologous molecules in a fashion similar to RAD51. BRCA2 interacts directly with both human RAD51 and DMC1; in the case of RAD51, this interaction results in stimulation of RAD51-promoted DNA strand exchange. However, for DMC1, little is known regarding the basis and functional cons...
18 CitationsSource
#1Theetha L. Pavankumar (UC Davis: University of California, Davis)H-Index: 3
#2J.C. Exell (UC Davis: University of California, Davis)H-Index: 1
Last.Stephen C. Kowalczykowski (UC Davis: University of California, Davis)H-Index: 76
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Abstract The unique translocation and DNA unwinding properties of DNA helicases can be concealed by the stochastic behavior of enzyme molecules within the necessarily large populations used in ensemble experiments. With recent technological advances, the direct visualization of helicases acting on individual DNA molecules has contributed significantly to the current understanding of their mechanisms of action and biological functions. The combination of single-molecule techniques that enable bot...
2 CitationsSource
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