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Naoki Kunishima
Osaka University
320Publications
21H-index
2,728Citations
Publications 316
Newest
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Elena Pérez (UEM: European University of Madrid)H-Index: 2
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 6 authors...
Abstract The present work describes the functional and structural characterization of adenine phosphoribosyltransferase 2 from Thermus thermophilus HB8 (TtAPRT2). The combination of structural and substrate specificity data provided valuable information for immobilization studies. Dimeric TtAPRT2 was immobilized onto glutaraldehyde-activated MagReSyn®Amine magnetic iron oxide porous microparticles by two different strategies: a) an enzyme immobilization at pH 8.5 to encourage the immobilization ...
2 CitationsSource
Abstract It is known that the crystallizability of protein molecules may be improved by replacing their surface lysine residues with other residue types. Here an experimental method to identify surface lysine residues by NHS-biotin chemical modification combined with MALDI-TOF MS was proposed and was evaluated using PH1033 protein from Pyrococcus horikoshii . Interestingly, the biotinylation experiment with a protein-reagent molar ratio of 1:1 revealed that only seven of twenty-two lysine residu...
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#1Yoshinori MatsuuraH-Index: 8
#2Michiyo TakehiraH-Index: 7
Last.Katsuhide YutaniH-Index: 37
view all 7 authors...
In order to elucidate the contribution of charged residues to protein stabilization at temperatures of over 100 °C, we constructed many mutants of the CutA1 protein (EcCutA1) from Escherichia coli. The goal was to see if one can achieve the same stability as for a CutA1 from hyperthermophile Pyrococcus horikoshii that has the denaturation temperature near 150 °C. The hydrophobic mutant of EcCutA1 (Ec0VV) with denaturation temperature (Td) of 113.2 °C was used as a template for mutations. The hig...
1 CitationsSource
#1Jon Del Arco (UEM: European University of Madrid)H-Index: 4
#2Javier Acosta (UEM: European University of Madrid)H-Index: 2
Last.Jesús Fernández-Lucas (Grupo México)H-Index: 12
view all 7 authors...
The use of enzymes as biocatalysts applied to synthesis of modified nucleoside-5'-monophosphates (NMPs) is an interesting alternative to traditional multistep chemical methods which offers several advantages, such as stereo, regio and enantioselectivity, simple downstream processing, and mild reaction conditions. Herein we report the recombinant expression, production and purification of uracil phosphoribosyltransferase from Thermus themophilus HB8 (TtUPRT). The structure of TtUPRT has been dete...
5 CitationsSource
#1Kazunori D. Yamada (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 10
#2Naoki KunishimaH-Index: 21
Last.Kentaro Tomii (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 20
view all 7 authors...
An alternative rational approach to improve protein crystals by using single-site mutation of surface residues is proposed based on the results of a statistical analysis using a compiled data set of 918 independent crystal structures, thereby reflecting not only the entropic effect but also other effects upon protein crystallization. This analysis reveals a clear difference in the crystal-packing propensity of amino acids depending on the secondary-structural class. To verify this result, a syst...
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#1Kazunori D. YamadaH-Index: 10
#2Naoki KunishimaH-Index: 21
Last.Kentaro TomiiH-Index: 20
view all 7 authors...
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#1Kazunori D. YamadaH-Index: 10
#2Naoki KunishimaH-Index: 21
Last.Kentaro TomiiH-Index: 20
view all 7 authors...
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#1Naoki KunishimaH-Index: 21
#2Hisashi NaitowH-Index: 10
Last.Yoshinori MatsuuraH-Index: 8
view all 3 authors...
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#1Naoki KunishimaH-Index: 21
#2Hisashi NaitowH-Index: 10
Last.Yoshinori MatsuuraH-Index: 8
view all 3 authors...
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#1Naoki KunishimaH-Index: 21
#2Hisashi NaitowH-Index: 10
Last.Yoshinori MatsuuraH-Index: 8
view all 3 authors...
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