#2Zeran Li(WashU: Washington University in St. Louis)H-Index: 2
Last.Kristy Bergmann(WashU: Washington University in St. Louis)H-Index: 1
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Alzheimer’s disease (AD) is the most common form of dementia. This neurodegenerative disorder is associated with neuronal death and gliosis heavily impacting the cerebral cortex. AD has a substantial but heterogeneous genetic component, presenting both Mendelian and complex genetic architectures. Using bulk RNA-seq from the parietal lobes and deconvolution methods, we previously reported that brains exhibiting different AD genetic architecture exhibit different cellular proportions. Here, we sou...
#2Bruno A. Benitez(WashU: Washington University in St. Louis)H-Index: 15
Last.Shan Jiang(WashU: Washington University in St. Louis)H-Index: 1
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Background Low frequency coding variants in TREM2 are associated with Alzheimer disease (AD) risk and cerebrospinal fluid (CSF) TREM2 protein levels are different between AD cases and controls. Similarly, TREM2 risk variant carriers also exhibit differential CSF TREM2 levels. TREM2 has three different alternative transcripts, but most of the functional studies only model the longest transcript. No studies have analyzed TREM2 expression levels or alternative splicing in brains from AD and cogniti...
#1Zeran Li(WashU: Washington University in St. Louis)H-Index: 2
#2F. G. Farias(WashU: Washington University in St. Louis)H-Index: 1
Last.Allison M. Lake(Vandy: Vanderbilt University)H-Index: 5
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Apart from amyloid β deposition and tau neurofibrillary tangles, Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neuronal loss and astrocytosis in the cerebral cortex. The goal of this study is to investigate genetic factors associated with the neuronal proportion in health and disease. To identify cell-autonomous genetic variants associated with neuronal proportion in cortical tissues, we inferred cellular population structure from bulk RNA-Seq derived from 1536 indivi...
#2Zeran Li(WashU: Washington University in St. Louis)H-Index: 2
Last.Fengxian Wang(WashU: Washington University in St. Louis)
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Alzheimer Disease (AD) is the most common form of dementia. This neurodegenerative disorder is associated with neuronal death and gliosis specifically in the cerebral cortex. AD has a substantial but heterogeneous genetic component, presenting both Mendelian and complex genetic architectures. We previously reported different cellular proportions between brains exhibiting different AD genetic architecture, that we identified using bulk RNA-seq from homogenized parietal lobes and deconvolution met...
Last.John Budde(WashU: Washington University in St. Louis)H-Index: 21
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Background: In previous studies, we observed decreased neuronal and increased astrocyte proportions in AD cases in parietal brain cortex by using a deconvolution method for bulk RNA-seq. These findings suggested that genetic risk factors associated with AD etiology have a specific effect in the cellular composition of AD brains. The goal of this study is to investigate if there are genetic determinants for brain cell compositions. Methods: Using cell type composition inferred from transcriptome ...
Last.Oscar Harari(WashU: Washington University in St. Louis)H-Index: 13
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Purpose of the Review This review summarizes the current state of the art of polygenic risk scores (PRSs) in the assessment of risk for neurodegenerative diseases.
#1Yanhua Zhou(WashU: Washington University in St. Louis)H-Index: 32
#2Kristine M. Wylie(WashU: Washington University in St. Louis)H-Index: 23
Last.George M. Weinstock(UMMC: University of Mississippi Medical Center)H-Index: 98
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ABSTRACT The potential to rapidly capture the entire microbial community structure and/or gene content makes metagenomic sequencing an attractive tool for pathogen identification and the detection of resistance/virulence genes in clinical settings. Here, we assessed the consistency between PCR from a diagnostic laboratory, quantitative PCR (qPCR) from a research laboratory, 16S rRNA gene sequencing, and metagenomic shotgun sequencing (MSS) for Clostridium difficile identification in diarrhea sto...
Background Resistant starch (RS) decreases intestinal inflammation in some settings. We tested the hypothesis that gut inflammation will be reduced with dietary supplementation with RS in rural Malawian children. Eighteen stunted 3–5-year-old children were supplemented with 8.5 g/day of RS type 2 for 4 weeks. The fecal samples were analyzed for the microbiota, the microbiome, short chain fatty acids, metabolome, and proteins indicative of inflammation before and after the intervention. Subjects ...
Last.George M. Weinstock(WashU: Washington University in St. Louis)H-Index: 98
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Background: The Human Microbiome Project (HMP) was undertaken with the goal of defining microbial communities in and on the bodies of healthy individuals using high-throughput metagenomic sequencing analysis. The viruses present in these microbial communities, the ‘human virome,’ are an important aspect of the human microbiome that is particularly understudied in the absence of overt disease. We analyzed eukaryotic double-stranded DNA (dsDNA) viruses, together with dsDNA replicative intermediate...
Last.Erica Sodergren(University of Connecticut Health Center)H-Index: 11
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Background Determining bacterial abundance variation is the first step in understanding bacterial similarity between individuals. Categorization of bacterial communities into groups or community classes is the subsequent step in describing microbial distribution based on abundance patterns. Here, we present an analysis of the groupings of bacterial communities in stool, nasal, skin, vaginal and oral habitats in a healthy cohort of 236 subjects from the Human Microbiome Project.