Takayuki Arazoe
Tokyo University of Science
Publications 15
#1Tohru Yamato (Meiji University)H-Index: 2
#2Ai Handa (Meiji University)
Last.Shigeru Kuwata (Meiji University)H-Index: 8
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Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing has become a promising approach for efficient and versatile genetic engineering in various organisms; however, simple and precise nucleotide modification methods in filamentous fungi have been restricted to double crossover type homologous recombination (HR). In this study, we developed a novel genome editing strategy via single crossover-mediated HR in the model filament...
#1Akihito Nozaka (Ridai: Tokyo University of Science)H-Index: 1
#2Ayaka Nishiwaki (Ridai: Tokyo University of Science)
Last.Hayao Taguchi (Ridai: Tokyo University of Science)H-Index: 17
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Chloramphenicol (Cm) is a broad-spectrum classic antibiotic active against prokaryotic organisms. However, Cm has severe side effects in eukaryotes of which the cause remains unknown. The plant pathogenic fungus Magnaporthe oryzae, which causes rice blast, forms an appressorium to infect the host cell via single-cell differentiation. Chloramphenicol specifically inhibits appressorium formation, which indicates that Cm has a novel molecular target (or targets) in the rice blast fungus. Applicatio...
#1Satomi Banno (Kobe University)H-Index: 2
#2Keiji Nishida (Kobe University)H-Index: 23
Last.Akihiko Kondo (Kobe University)H-Index: 68
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In eukaryotes, the CRISPR–Cas9 system has now been widely used as a revolutionary genome engineering tool1, 2. However, in prokaryotes, the use of nuclease-mediated genome editing tools has been limited to negative selection for the already modified cells because of its lethality3, 4. Here, we report on deaminase-mediated targeted nucleotide editing (Target-AID) 5 adopted in Escherichia coli. Cytidine deaminase PmCDA1 fused to the nuclease-deficient CRISPR–Cas9 system achieved specific point mut...
#1Hiroya Itoh (Astellas Pharma)H-Index: 2
#2Ai Miura (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 4
Last.Takashi Shibata (Astellas Pharma)H-Index: 3
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In the production of useful microbial secondary metabolites, the breeding of strains is generally performed by random mutagenesis. However, because random mutagenesis introduces many mutations into genomic DNA, the causative mutations leading to increased productivity are mostly unknown. Therefore, although gene targeting is more efficient for breeding than random mutagenesis, it is difficult to apply. In this study, a wild-type strain and randomly mutagenized strains of fungal sp. No. 14919, a ...
#1Zenpei ShimataniH-Index: 9
#2Sachiko KashojiyaH-Index: 2
Last.Kenji MiuraH-Index: 31
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Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion).
#1Osamu MizutaniH-Index: 11
#2Takayuki Arazoe (Meiji University)H-Index: 6
Last.Osamu YamadaH-Index: 19
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Transcription activator-like effector nucleases (TALENs), which can generate DNA double-strand breaks at specific sites in the desired genome locus, have been used in many organisms as a tool for genome editing. In Aspergilli, including Aspergillus oryzae , however, the use of TALENs has not been validated. In this study, we performed genome editing of A . oryzae wild-type strain via error of nonhomologous end-joining (NHEJ) repair by transient expression of high-efficiency Platinum-Fungal TALEN...
#1Keiji Nishida (Kobe University)H-Index: 23
#2Takayuki Arazoe (Kobe University)H-Index: 6
Last.Kiyotaka Y. Hara (University of Shizuoka)H-Index: 17
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INTRODUCTION To combat invading pathogens, cells develop an adaptive immune response by changing their own genetic information. In vertebrates, the generation of genetic variation (somatic hypermutation) is an essential process for diversification and affinity maturation of antibodies that function to detect and sequester various foreign biomolecules. The activation-induced cytidine deaminase (AID) carries out hypermutation by modifying deoxycytidine bases in the variable region of the immunoglo...