Thanos Tzounopoulos
University of Pittsburgh
Publications 33
#1Chung Yang Yeh (University of Pittsburgh)H-Index: 1
#2Zhaofeng Ye (THU: Tsinghua University)H-Index: 2
Last.Carlos J. Camacho (University of Pittsburgh)H-Index: 35
view all 12 authors...
The neuronal cell death-promoting loss of cytoplasmic K+ following injury is mediated by an increase in Kv2.1 potassium channels in the plasma membrane. This phenomenon relies on Kv2.1 binding to syntaxin 1A via 9 amino acids within the channel intrinsically disordered C terminus. Preventing this interaction with a cell and blood-brain barrier-permeant peptide is neuroprotective in an in vivo stroke model. Here a rational approach was applied to define the key molecular interactions between synt...
#1Ruiting Liu (University of Pittsburgh)H-Index: 1
#2Thanos Tzounopoulos (University of Pittsburgh)H-Index: 15
Last.Peter Wipf (University of Pittsburgh)H-Index: 70
view all 3 authors...
Based on the potent Kv7 agonist RL-81, we prepared new lead structures with greatly improved selectivity for Kv7.2/Kv7.3 over related potassium channels, i.e., Kv7.3/Kv7.5, Kv7.4, and Kv7.4/7.5. RL-36 and RL-12 maintain an agonist EC2x of ca. 1 μM on Kv7.2/Kv7.3 in a high-throughput assay on an automated electrophysiology platform in HEK293 cells but lack activity on Kv7.3/Kv7.5, Kv7.4, and Kv7.4/7.5, resulting in a selectivity index SI > 10. RL-56 is remarkably potent, EC2x 0.11 ± 0.02 μM, and ...
#1Thanos Tzounopoulos (University of Pittsburgh)H-Index: 15
#2Carey D. Balaban (University of Pittsburgh)H-Index: 44
Last.Catherine V. Palmer (University of Pittsburgh)H-Index: 15
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In this position review, we propose to establish a path for replacing the empirical classification of tinnitus with a taxonomy from precision medicine. The goal of a classification system is to understand the inherent heterogeneity of individuals experiencing and suffering from tinnitus and to identify what differentiates potential subgroups. Identification of different patient subgroups with distinct audiological, psychophysical, and neurophysiological characteristics will facilitate the manage...
1 CitationsSource
#1Manoj Kumar (University of Pittsburgh)H-Index: 2
#2Shanshan Xiong (CSU: Central South University)H-Index: 2
Last.Charles T. Anderson (University of Pittsburgh)H-Index: 13
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Neurons in the auditory cortex are tuned to specific ranges of sound frequencies. Although the cellular and network mechanisms underlying neuronal sound frequency selectivity are well-studied and reflect the interplay of thalamocortical and intracortical excitatory inputs and further refinement by cortical inhibition, the precise synaptic signaling mechanisms remain less understood. To gain further understanding on these mechanisms and their effects on sound-driven behavior, we employed in vivo ...
2 CitationsSource
#1Jacob M. Goldberg (MIT: Massachusetts Institute of Technology)H-Index: 12
#2Fang Wang (MIT: Massachusetts Institute of Technology)H-Index: 20
Last.Stephen J. Lippard (MIT: Massachusetts Institute of Technology)H-Index: 119
view all 8 authors...
Fluorescent sensors for mobile zinc are valuable for studying complex biological systems. Because these sensors typically bind zinc rapidly and tightly, there has been little temporal control over the activity of the probe after its application to a sample. The ability to control the activity of a zinc sensor in vivo during imaging experiments would greatly improve the time resolution of the measurement. Here, we describe photoactivatable zinc sensors that can be triggered with short pulses of U...
22 CitationsSource
#1Charles T. Anderson (University of Pittsburgh)H-Index: 13
#2Manoj Kumar (University of Pittsburgh)H-Index: 2
Last.Thanos Tzounopoulos (University of Pittsburgh)H-Index: 15
view all 4 authors...
Many people find it easy to follow a conversation while on a busy city street, but this seemingly simple task requires sophisticated processing of sounds. The brain must accurately distinguish speech sounds from background noise, even though the volumes and pitches of those sounds overlap. To make this possible, neurons that process sounds continually adjust the relationship between the volume of a sound and the size of their response. This helps the brain to distinguish more precisely between d...
9 CitationsSource
#1Charles T. Anderson (University of Pittsburgh)H-Index: 13
#2Manoj Kumar (University of Pittsburgh)H-Index: 2
Last.Thanos Tzounopoulos (University of Pittsburgh)H-Index: 15
view all 4 authors...
#1Chung Yang YehH-Index: 1
#2Ashlyn M. BulasH-Index: 1
Last.Elias AizenmanH-Index: 47
view all 10 authors...
National Institutes of Health [NS043277, DC007905, 5T32NS007433-18]; American Heart Association [16PRE29170009]
6 CitationsSource
#1Bopanna I. Kalappa (University of Pittsburgh)H-Index: 9
#2Thanos Tzounopoulos (University of Pittsburgh)H-Index: 15
Abstract Synaptically released zinc inhibits baseline excitatory neurotransmission; however, the role of this neuromodulator on short-term plasticity during different levels of synaptic activity remains largely unknown. This lack of knowledge prevents our understanding of information transfer across zinc-releasing synapses, including 50% of excitatory synapses in cortical areas. We used in vitro electrophysiology in mouse brain slices and discovered that the effects of zinc on excitatory postsyn...
6 CitationsSource
#1Cesar C. Ceballos (USP: University of São Paulo)H-Index: 5
#2Shuang Li (University of Pittsburgh)H-Index: 6
Last.Ricardo M. Leão (USP: University of São Paulo)H-Index: 17
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In a neuronal population, several combinations of its ionic conductances are used to attain a specific firing phenotype. Some neurons present heterogeneity in their firing, generally produced by expression of a specific conductance, but how additional conductances vary along in order to homeostatically regulate membrane excitability is less known. Dorsal cochlear nucleus principal neurons, fusiform neurons, display heterogeneous spontaneous action potential activity and thus represent an appropr...
7 CitationsSource