Junji Fujikura
Kyoto University
EndocrinologyAdipose tissueInsulinDiabetes mellitusBiology
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Publications 52
#1Toshihiro Nakamura (Kyoto University)H-Index: 10
#1Toshihiro Nakamura (Kyoto University)H-Index: 23
Last. Nobuya Inagaki (Kyoto University)H-Index: 59
view all 8 authors...
#1Takaaki MurakamiH-Index: 2
#2Hiroyuki FujimotoH-Index: 38
Last. Nobuya InagakiH-Index: 59
view all 6 authors...
#1Nobuko Yamada-Goto (Kyoto University)H-Index: 3
#2Yukari Ochi (Kyoto University)H-Index: 4
Last. Kazuwa Nakao (Kyoto University)H-Index: 117
view all 12 authors...
Abstract Background The preparation of human neurons derived from human induced pluripotent stem (iPS) cells can serve as a potential tool for evaluating the physiological and pathophysiological properties of human neurons and for drug development. Methods In the present study, the functional activity in neuronal cells differentiated from human iPS cells was observed. Results The differentiated cells expressed mRNAs for classical neuronal markers (microtubule-associated protein 2, β-tubulin III,...
#1Shoko Ohno (Kyoto University)H-Index: 5
#2Hideki Yokoi (Kyoto University)H-Index: 19
Last. Masashi Mukoyama (Kumamoto University)H-Index: 17
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Pharmacological blockade of the N- and L-type calcium channel lessens renal injury in kidney disease patients. The significance of specific blockade of α1 subunit of N-type calcium channel, Cav2.2, in diabetic nephropathy, however, remains to be clarified. To examine functional roles, we mated Cav2.2−/− mice with db/db (diabetic) mice on the C57BLKS background. Cav2.2 was localized in glomeruli including podocytes and in distal tubular cells. Diabetic Cav2.2−/− mice significantly reduced urinary...
11 CitationsSource
#1Eisaku Mori (Kyoto University)H-Index: 6
#2Junji Fujikura (Kyoto University)H-Index: 23
Last. Kazuwa Nakao (Kyoto University)H-Index: 117
view all 15 authors...
Abstract Objective Congenital generalized lipodystrophy (CGL) is an autosomal recessive disorder characterized by marked scarcity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early-onset diabetes. Mutation of the BSCL2 / SEIPIN gene causes the most severe form of CGL. The aim of this study was to generate induced pluripotent stem (iPS) cells from patients with CGL harboring BSCL2 / SEIPIN mutations. Methods Skin biopsies were obtained from two Japane...
13 CitationsSource
#1Takeru Sakai (Kyoto University)H-Index: 6
#2Toru Kusakabe (Kyoto University)H-Index: 15
Last. Kazuwa Nakao (Kyoto University)H-Index: 117
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Leptin may reduce pancreatic lipid deposition, which increases with progression of obesity and can impair β-cell function. The insulinotropic effect of glucagon-like peptide-1 (GLP-1) and the efficacy of GLP-1 receptor agonist are reduced associated with impaired β-cell function. In this study, we examined whether leptin could restore the efficacy of exenatide, a GLP-1 receptor agonist, in type 2 diabetes with increased adiposity. We chronically administered leptin (500 μg·kg−1·day−1) and/or exe...
9 CitationsSource
#1Tsutomu Tomita (Kyoto University)H-Index: 18
#2Kiminori Hosoda (Kyoto University)H-Index: 59
Last. Kazuwa Nakao (Kyoto University)H-Index: 117
view all 5 authors...
Free fatty acids (FFAs) play a pivotal role in metabolic control and cell signaling processes in various tissues. In particular, FFAs are known to augment glucose-stimulated insulin secretion (GSIS) by pancreatic beta cells, where fatty acid-derived metabolites, such as long chain fatty acyl-CoAs, are believed to act as crucial effectors. Recently, G-protein-coupled receptor 40 (GPR40), a receptor for long-chain fatty acids, was reported to be highly expressed in pancreatic beta cells and involv...
18 CitationsSource
#1Michio NoguchiH-Index: 14
#2Kiminori HosodaH-Index: 59
Last. Kazuwa NakaoH-Index: 117
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Human induced pluripotent stem (iPS) and embryonic stem (ES) cells can differentiate into a variety of cell types. We reported on adipogenic potential of human iPS and ES cells in vitro. In the present study, we investigate the survival and maintenance of adipocytes differentiated in vitro from human iPS and ES cells after transplantation. Following adipogenic induction in vitro, the differentiated cells exhibited functional properties of adipocytes such as lipid storage, lipolysis, and insulin ...
16 CitationsSource
#1Midori Ida (Kyoto University)H-Index: 2
#2Masakazu Hirata (Kyoto University)H-Index: 27
Last. Kazuwa Nakao (Kyoto University)H-Index: 117
view all 10 authors...
Objective To elucidate early change of intra-abdominal fat in response to calorie restriction in patients with obesity by weekly evaluation using a dual bioelectrical impedance analysis (Dual BIA) instrument. Methods For 67 Japanese patients with obesity, diabetes, or metabolic syndrome, intra-abdominal fat area (IAFA), initially with both Dual BIA and computed tomography (CT), and in subsequent weeks of calorie restriction, with Dual BIA were measured. Results IAFA by Dual BIA (Dual BIA-IAFA) c...
22 CitationsSource