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Larysa V. Yuzefovych
University of South Alabama
EndocrinologyMitochondrial DNAOxidative stressInsulin resistanceMitochondrion
12Publications
7H-index
480Citations
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Publications 12
Newest
#2Viktor PastukhH-Index: 14
Last. Lyudmila I. RachekH-Index: 16
view all 6 authors...
Cells damaged by mechanical or infectious injury release proinflammatory mitochondrial DNA (mtDNA) fragments into the circulation. We evaluated the relation between plasma levels of mtDNA fragments in obese type 2 diabetes mellitus (T2DM) patients and measures of chronic inflammation and insulin resistance. In 10 obese T2DM patients and 12 healthy control (HC) subjects, we measured levels of plasma cell-free mtDNA with quantitative real-time polymerase chain reaction, and mtDNA damage in skeleta...
1 CitationsSource
Mitochondrial DNA (mtDNA) damage heightens mitochondrial oxidative stress, which is very critical for the development of insulin resistance. OGG1 (8-oxoguanine DNA glycosylase-1) is a DNA glycosylase mediating the first step in the base excision repair which removes 7,8-dihydro-8-oxoguanine (8-oxoG) and repairs oxidized nuclear and mtDNA. Previous studies showed that Ogg1 deficiency results in an increased susceptibility to high fat diet (HFD)-induced obesity, metabolic dysfunction and IR in mic...
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#2Viktor PastukhH-Index: 14
Last. Lyudmila I. RachekH-Index: 16
view all 3 authors...
Increasing evidence showed that mitochondrial DNA (mtDNA), as a part of mitochondrial damage-associated molecular patterns (DAMPs), induces an immune response and inflammation in immune, as well as in cells of nonnimmune origin. As insulin resistance and type 2 diabetes are characterized by a persistent chronic inflammation, the aim if this study was to evaluate if mtDNA DAMPs induce an inflammatory responses in skeletal muscle cells and induce insulin resistance both in vitro and vivo . In vitr...
2 CitationsSource
#2Michele SchulerH-Index: 1
Last. Lyudmila I. RachekH-Index: 16
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Mitochondrial DNA (mtDNA) damage has been implicated in the development of insulin resistance. The mtDNA is highly specialized and encodes for proteins essential for energy metabolism. Also, mtDNA damage heightens mitochondrial oxidative stress, which is very critical for insulin resistance. OGG1 (8-oxoguanine DNA glycosylase-1) is a DNA glycosylase mediating the first step in the base excision repair which removes 7,8-dihydro-8-oxoguanine (8-oxoG) and repairs oxidized nuclear and mitochondrial ...
Source
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Andrea G. Kahn (USA: University of South Alabama)H-Index: 5
Last. Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 16
view all 8 authors...
Production of mitochondrial reactive oxygen species and integrity of mitochondrial DNA (mtDNA) are crucial in breast cancer progression and metastasis. Therefore, we evaluated the role of mtDNA damage in breast cancer by genetically modulating the DNA repair enzyme 8-oxoguanine DNA glycosylase (OGG1) in the PyMT transgenic mouse model of mammary tumorigenesis. We generated mice lacking OGG1 (KO), mice overexpressing human OGG1 subunit 1alpha in mitochondria (Tg), and mice simultaneously lacking ...
15 CitationsSource
#2Susan P. LeDouxH-Index: 40
Last. Lyudmila I. RachekH-Index: 16
view all 4 authors...
Source
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Susan P. LeDoux (USA: University of South Alabama)H-Index: 40
Last. Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 16
view all 4 authors...
Saturated free fatty acids (FFAs) have been implicated in the increase of oxidative stress, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, autophagy, and insulin resistance (IR) observed in skeletal muscle. Previously, we have shown that palmitate-induced mitochondrial DNA (mtDNA) damage triggers mitochondrial dysfunction, mitochondrial reactive oxygen species (mtROS) production, apoptosis and IR in L6 myotubes. The present study showed that mitochondrial overexpression of human 8...
31 CitationsSource
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2A. Michele Schuler (USA: University of South Alabama)H-Index: 3
Last. Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 16
view all 8 authors...
Recent evidence has linked mitochondrial dysfunction and DNA damage, increased oxidative stress in skeletal muscle, and insulin resistance (IR). The purpose of this study was to determine the role of the DNA repair enzyme, human 8-oxoguanine DNA glycosylase/apurinic/apyrimidinic lyase (hOGG1), on palmitate-induced mitochondrial dysfunction and IR in primary cultures of skeletal muscle derived from hind limb of ogg1−/− knockout mice and transgenic mice, which overexpress human (hOGG1) in mitochon...
15 CitationsSource
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Sergiy I. Musiyenko (USA: University of South Alabama)H-Index: 4
Last. Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 16
view all 4 authors...
Background Recent studies showed a link between a high fat diet (HFD)-induced obesity and lipid accumulation in non-adipose tissues, such as skeletal muscle and liver, and insulin resistance (IR). Although the mechanisms responsible for IR in those tissues are different, oxidative stress and mitochondrial dysfunction have been implicated in the disease process. We tested the hypothesis that HFD induced mitochondrial DNA (mtDNA) damage and that this damage is associated with mitochondrial dysfunc...
134 CitationsSource
#2Viktoriya Solodushko (USA: University of South Alabama)H-Index: 15
Last. Lyudmila I. RachekH-Index: 16
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Saturated free fatty acids have been implicated in the increase of oxidative stress, mitochondrial dysfunction, apoptosis, and insulin resistance seen in type 2 diabetes. The purpose of this study was to determine whether palmitate-induced mitochondrial DNA (mtDNA) damage contributed to increased oxidative stress, mitochondrial dysfunction, apoptosis, impaired insulin signaling, and reduced glucose uptake in skeletal muscle cells. Adenoviral vectors were used to deliver the DNA repair enzyme hum...
46 CitationsSource
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