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Larysa V. Yuzefovych
University of South Alabama
10Publications
7H-index
452Citations
Publications 10
Newest
Mitochondrial DNA (mtDNA) damage heightens mitochondrial oxidative stress, which is very critical for the development of insulin resistance. OGG1 (8-oxoguanine DNA glycosylase-1) is a DNA glycosylase mediating the first step in the base excision repair which removes 7,8-dihydro-8-oxoguanine (8-oxoG) and repairs oxidized nuclear and mtDNA. Previous studies showed that Ogg1 deficiency results in an increased susceptibility to high fat diet (HFD)-induced obesity, metabolic dysfunction and IR in mic...
#2Viktor PastukhH-Index: 15
Last.Lyudmila I. RachekH-Index: 13
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Increasing evidence showed that mitochondrial DNA (mtDNA), as a part of mitochondrial damage-associated molecular patterns (DAMPs), induces an immune response and inflammation in immune, as well as in cells of nonnimmune origin. As insulin resistance and type 2 diabetes are characterized by a persistent chronic inflammation, the aim if this study was to evaluate if mtDNA DAMPs induce an inflammatory responses in skeletal muscle cells and induce insulin resistance both in vitro and vivo . In vitr...
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Andrea G. Kahn (USA: University of South Alabama)H-Index: 5
Last.Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 13
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Production of mitochondrial reactive oxygen species and integrity of mitochondrial DNA (mtDNA) are crucial in breast cancer progression and metastasis. Therefore, we evaluated the role of mtDNA damage in breast cancer by genetically modulating the DNA repair enzyme 8-oxoguanine DNA glycosylase (OGG1) in the PyMT transgenic mouse model of mammary tumorigenesis. We generated mice lacking OGG1 (KO), mice overexpressing human OGG1 subunit 1alpha in mitochondria (Tg), and mice simultaneously lacking ...
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Susan P. LeDoux (USA: University of South Alabama)H-Index: 39
Last.Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 13
view all 4 authors...
Saturated free fatty acids (FFAs) have been implicated in the increase of oxidative stress, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, autophagy, and insulin resistance (IR) observed in skeletal muscle. Previously, we have shown that palmitate-induced mitochondrial DNA (mtDNA) damage triggers mitochondrial dysfunction, mitochondrial reactive oxygen species (mtROS) production, apoptosis and IR in L6 myotubes. The present study showed that mitochondrial overexpression of human 8...
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2A. Michele Schuler (USA: University of South Alabama)H-Index: 3
Last.Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 13
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Recent evidence has linked mitochondrial dysfunction and DNA damage, increased oxidative stress in skeletal muscle, and insulin resistance (IR). The purpose of this study was to determine the role of the DNA repair enzyme, human 8-oxoguanine DNA glycosylase/apurinic/apyrimidinic lyase (hOGG1), on palmitate-induced mitochondrial dysfunction and IR in primary cultures of skeletal muscle derived from hind limb of ogg1−/− knockout mice and transgenic mice, which overexpress human (hOGG1) in mitochon...
#1Larysa V. Yuzefovych (USA: University of South Alabama)H-Index: 7
#2Sergiy Musiyenko (USA: University of South Alabama)H-Index: 3
Last.Lyudmila I. Rachek (USA: University of South Alabama)H-Index: 13
view all 4 authors...
Background Recent studies showed a link between a high fat diet (HFD)-induced obesity and lipid accumulation in non-adipose tissues, such as skeletal muscle and liver, and insulin resistance (IR). Although the mechanisms responsible for IR in those tissues are different, oxidative stress and mitochondrial dysfunction have been implicated in the disease process. We tested the hypothesis that HFD induced mitochondrial DNA (mtDNA) damage and that this damage is associated with mitochondrial dysfunc...
#2Viktoriya Solodushko (USA: University of South Alabama)H-Index: 15
Last.Lyudmila I. RachekH-Index: 13
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Saturated free fatty acids have been implicated in the increase of oxidative stress, mitochondrial dysfunction, apoptosis, and insulin resistance seen in type 2 diabetes. The purpose of this study was to determine whether palmitate-induced mitochondrial DNA (mtDNA) damage contributed to increased oxidative stress, mitochondrial dysfunction, apoptosis, impaired insulin signaling, and reduced glucose uptake in skeletal muscle cells. Adenoviral vectors were used to deliver the DNA repair enzyme hum...
The type of free fatty acids (FFAs), saturated or unsaturated, is critical in the development of insulin resistance (IR), since the degree of saturation correlates with IR. We compared the effects of the saturated FFA palmitate, the unsaturated FFA oleate, and a mixture of each on the production of mitochondrial reactive oxygen species (mtROS), mitochondrial DNA (mtDNA) damage, mitochondrial function, apoptosis, and insulin-signaling pathway in skeletal muscle cells. Only palmitate caused a sign...
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