Fredrik Lanner
Karolinska Institutet
Publications 52
#1Eleni Gelali (Science for Life Laboratory)H-Index: 1
#2Gabriele Girelli (Science for Life Laboratory)H-Index: 1
Last.Reza Mirzazadeh (Science for Life Laboratory)H-Index: 1
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DNA fluorescence in situ hybridization (DNA FISH) is a powerful method to study chromosomal organization in single cells. At present, there is a lack of free resources of DNA FISH probes and probe design tools which can be readily applied. Here, we describe iFISH, an open-source repository currently comprising 380 DNA FISH probes targeting multiple loci on the human autosomes and chromosome X, as well as a genome-wide database of optimally designed oligonucleotides and a freely accessible web in...
#1Magdalena Wagner (KI: Karolinska Institutet)H-Index: 1
#2Magdalena Wagner (Karolinska University Hospital)
Last.Haojiang Lu (KI: Karolinska Institutet)
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The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. We analysed on a single cell level the transcriptome and cell surface antigen profiles of over 24,000 cells from hig...
#1Nicolás M Ortega (KI: Karolinska Institutet)
#2Nerges Winblad (KI: Karolinska Institutet)H-Index: 1
Last.Fredrik Lanner (KI: Karolinska Institutet)H-Index: 20
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Understanding the genetic underpinning of early human development is of great interest not only for basic developmental and stem cell biology but also for regenerative medicine, infertility treatments, and better understanding the causes of congenital disease. Our current knowledge has mainly been generated with the use of laboratory animals, especially the mouse. While human and mouse early development present morphological resemblance, we know that the timing of the events as well as the cellu...
#1Alvaro Plaza Reyes (KI: Karolinska Institutet)H-Index: 5
#2Fredrik Lanner (KI: Karolinska Institutet)H-Index: 20
Although endonuclease-mediated genome editing techniques offer significant improvement over traditional methods, they are still ineffective for introduction of large DNA sequences. Recently in Nature Biotechnology , Gu et al. (2018) developed a CRISPR-Cas strategy termed 2C-HR-CRISPR that generates fluorescent reporter tagging of genes with up to 95% knockin efficiency in mouse embryos.
#1Sandra Petrus-Reurer (KI: Karolinska Institutet)H-Index: 3
#2Hammurabi Bartuma (KI: Karolinska Institutet)H-Index: 4
Last.Anders Kvanta (KI: Karolinska Institutet)H-Index: 24
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Geographic atrophy (GA), the late stage of dry age-related macular degeneration is characterized by loss of the retinal pigment epithelial (RPE) layer, which leads to subsequent degeneration of vital retinal structures (e.g., photoreceptors) causing severe vision impairment. Similarly, RPE-loss and decrease in visual acuity is seen in long-term follow up of patients with advanced wet age-related macular degeneration (AMD) receiving intravitreal anti-vascular endothelial growth factor (VEGF) trea...
#1Camilla Mohlin (LNU: Linnaeus University)H-Index: 7
#2Sandra Petrus-Reurer (KI: Karolinska Institutet)H-Index: 3
Last.Kristina Nilsson-Ekdahl (Uppsala University)H-Index: 38
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Is the polarized secretion of complement factor H of importance in age-related macular degeneration?
#1Olga Tšuiko (UT: University of Tartu)H-Index: 2
#2Tatjana JatsenkoH-Index: 1
Last.Andres SalumetsH-Index: 24
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Abstract The journey of embryonic development starts at oocyte fertilization, which triggers a complex cascade of events and cellular pathways that guide early embryogenesis. Recent technological advances have greatly expanded our knowledge of cleavage-stage embryo development, which is characterized by an increased rate of whole-chromosome losses and gains, mixoploidy, and atypical cleavage morphokinetics. Embryonic aneuploidy significantly contributes to implantation failure, spontaneous misca...