Jacques J.M. van Dongen
Erasmus University Rotterdam
Molecular biologyImmunologyMinimal residual diseaseMedicineBiology
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Publications 589
#1Laura Garcia-Perez (LUMC: Leiden University Medical Center)H-Index: 2
#2Marja C.J.A. van Eggermond (LUMC: Leiden University Medical Center)H-Index: 17
Last. Marina Cavazzana-Calvo (Paris V: Paris Descartes University)H-Index: 74
view all 22 authors...
Abstract Recombinase-activating gene-1 (RAG1) deficient SCID patients lack B and T lymphocytes due to the inability to rearrange immunoglobulin and T-cell receptor genes. Gene therapy is an alternative for those RAG1-SCID patients who lack a suitable bone marrow donor. We designed lentiviral vectors with different internal promoters driving codon optimized RAG1 to ensure optimal expression. We used Rag1-/- mice as preclinical model for RAG1-SCID to assess the efficacy of the various vectors. We ...
1 CitationsSource
#1Christina Grosserichter-Wagener (EUR: Erasmus University Rotterdam)H-Index: 6
#2Alexander Franco-Gallego (UdeA: University of Antioquia)
Last. Marco W.J. Schreurs (EUR: Erasmus University Rotterdam)H-Index: 14
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Objective: Selective IgA deficiency (sIgAD) is the most common primary immunodeficiency in Western countries. Patients can suffer from recurrent infections and autoimmune diseases because of a largely unknown aetiology. To increase insights into the pathophysiology of the disease, we studied memory B and T cells and cytokine concentrations in peripheral blood. Methods: We analysed 30 sIgAD patients (12 children, 18 adults) through detailed phenotyping of peripheral B-cell, CD8(+) T-cell and CD4(...
#1Indu Khatri (LUMC: Leiden University Medical Center)H-Index: 8
#2Magdalena A. Berkowska (LUMC: Leiden University Medical Center)H-Index: 2
Last. Jacques J.M. van Dongen (LUMC: Leiden University Medical Center)H-Index: 92
view all 6 authors...
At the population level, immunoglobulin (IG) loci harbor inter-individual allelic variants in the many different germline IG variable (V), Diversity (D) and Joining (J) genes of the IG heavy (IGH), IG kappa (IGK) and IG lambda (IGL) loci, which together form the genetic basis of the highly diverse antigen-specific B-cell receptors. These inter-individual allelic variants can be shared between or be specific to human populations. The current IG databases IMGT, VBASE2 and IgPdb hold information ab...
1 CitationsSource
#1Tomas Kalina (Charles University in Prague)H-Index: 23
#2Marina Bakardjieva (Charles University in Prague)H-Index: 1
Last. Ingrid Pico-Knijnenburg (EUR: Erasmus University Rotterdam)H-Index: 9
view all 24 authors...
The EuroFlow PID consortium developed a set of flow cytometry tests for evaluation of patients with suspicion of primary immunodeficiency (PID). In this technical report we evaluate the performance of the SCID-RTE tube that explores the presence of recent thymic emigrants (RTE) together with T-cell activation status and maturation stages and discuss its applicability in the context of the broader EuroFlow PID flow cytometry testing algorithm for diagnostic orientation of PID of the lymphoid syst...
#1Bruno Paiva (University of Navarra)H-Index: 40
#2Noemi PuigH-Index: 21
Last. Juan José LahuertaH-Index: 39
view all 35 authors...
PURPOSE Assessing measurable residual disease (MRD) has become standard with many tumors, but the clinical meaning of MRD in multiple myeloma (MM) remains uncertain, particularly when assessed by n...
6 CitationsSource
#1Vitor BotafogoH-Index: 2
#2Martin Perez-Andres (University of Salamanca)H-Index: 17
Last. Alfonso RomeroH-Index: 10
view all 22 authors...
CD4+ T-cells comprise multiple functionally distinct cell populations that play a key role in immunity. Despite blood monitoring of CD4+ T-cell subsets is of potential clinical utility, no standardized and validated approaches have been proposed so far. The aim of this study was to design and validate a single 14-color antibody combination for sensitive and reproducible flow-cytometry monitoring of CD4+ T-cell populations in human blood to establish normal age-related reference values and evalua...
3 CitationsSource
#1Wouter van den Bossche (EUR: Erasmus University Rotterdam)H-Index: 5
#2Kyrill RykovH-Index: 2
Last. Jos J. A. M. van RaayH-Index: 15
view all 11 authors...
The authors regret not to have included the appropriate reference to the support by the European Research Council. The presented research was funded by the European Research Council under the European Union's Horizon 2020 Research and Innovation Programme: grant agreement ERC-2015-AdG 695655, TiMaScan. The authors would like to apologise for any inconvenience caused.
#1Ewa BernatowskaH-Index: 19
Last. Bożena Mikołuć (UMB: Medical University of Białystok)H-Index: 10
view all 24 authors...
Objectives The aim of the study was to estimate the rate of adverse reactions to live BCG Moreau vaccine, manufactured by Biomed in Poland, in severe combined immunodeficiency (SCID) patients.
#1Ruud W. J. Meijers (EUR: Erasmus University Rotterdam)H-Index: 10
#2Alice F. Muggen (EUR: Erasmus University Rotterdam)H-Index: 6
Last. Anton W. Langerak (EUR: Erasmus University Rotterdam)H-Index: 53
view all 7 authors...
Chronic lymphocytic leukemia is a heterogeneous disease based on clinical and biological characteristics. Differences in Ca2+-levels among cases, both basal and upon B-cell receptor stimulation may reflect heterogeneity in the pathogenesis due to cell-intrinsic factors. Our aim is to elucidate cell-intrinsic differences between B-cell receptor responsive and unresponsive cases. Therefore the B-cell receptor responsiveness was determined ex vivo based on Ca2+-influx upon α-IgM stimulation of puri...
1 CitationsSource
#1Luzalba Sanoja-Flores (University of Salamanca)H-Index: 5
Last. Alberto Orfao (University of Salamanca)H-Index: 68
view all 24 authors...
5 CitationsSource