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Andrew J. Simkin
University of Essex
GeneGeneticsBiochemistryBotanyBiology
51Publications
24H-index
2,449Citations
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Publications 53
Newest
#1Andrew J. SimkinH-Index: 24
#2Michele Faralli (University of Essex)H-Index: 1
Last. Tracy Lawson (University of Essex)H-Index: 38
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Photosynthesis is currently a focus for crop improvement, however the majority of this work has taken place and been assessed in leaves, whilst limited consideration has been given to the contribution that other green tissues make to whole plant carbon assimilation. The major focus of this review is to evaluate the impact of non‐foliar photosynthesis on carbon use efficiency and total assimilation. Here we appraise and summarise past and current literature on the substantial contribution of diff...
2 CitationsSource
#1Patricia E. Lopez-Calcagno (University of Essex)H-Index: 7
#2Kenny Lee BrownH-Index: 1
Last. Christine A. RainesH-Index: 40
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Previous studies have demonstrated that independent stimulation of either electron transport or RuBP regeneration can increase the rate of photosynthetic carbon assimilation and plant biomass. In this paper, we present evidence that a multi-gene approach to simultaneously manipulate these two processes provides a further stimulation of photosynthesis. We report on the introduction of the cyanobacterial bifunctional enzyme fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase or overexpres...
Source
#1Andrew J. SimkinH-Index: 24
Increasing demands for food and resources are challenging existing markets, driving a need to continually investigate and establish crop varieties with improved yields and health benefits. By the later part of the century, current estimates indicate that a >50% increase in the yield of most of the important food crops including wheat, rice and barley will be needed to maintain food supplies and improve nutritional quality to tackle what has become known as ‘hidden hunger’. Improving the nutritio...
2 CitationsSource
#1Fiona WilsonH-Index: 3
#2Kate HarrisonH-Index: 1
Last. Richard J. HarrisonH-Index: 14
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Background Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recently Fragaria. In order to inform application of the technology in Fragaria, we targeted the visible endogenous marker gene PDS (phytoene desaturase) in diploid Fragaria vesca ssp. vesca ‘Hawaii 4’ and octoploid F. × ananassa ‘Calypso’.
5 CitationsSource
#1Andrew J. Simkin (University of Essex)H-Index: 24
#2Patricia E. Lopez-Calcagno (University of Essex)H-Index: 7
Last. Christine A. Raines (University of Essex)H-Index: 40
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A number of recent studies have provided strong support demonstrating that improving the photosynthetic processes through genetic engineering can provide an avenue to improve yield potential. The major focus of this review is on improvement of the Calvin–Benson cycle and electron transport. Consideration is also given to how altering regulatory process may provide an additional route to increase photosynthetic efficiency. Here we summarize some of the recent successes that have been observed thr...
11 CitationsSource
#1Saqer AlotaibiH-Index: 2
#2Hatim AlyasiH-Index: 1
Last. Christine A. RainesH-Index: 40
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1 CitationsSource
#1Saqer AlotaibiH-Index: 2
#2Caroline A. SparksH-Index: 18
Last. Christine A. RainesH-Index: 40
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Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bis...
2 CitationsSource
Last. Andrew J. SimkinH-Index: 24
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Gateway technology has been used to facilitate the generation of a large number of constructs for the modification of plants for research purposes. However, many of the currently available vectors only allow the integration of a single cDNA of interest into an expression clone. The ability to over-express multiple genes in combination is essential for the study of plant development where several transcripts have a role to play in one or more metabolic processes. The tools to carry out such studi...
2 CitationsSource
#1Steven M. Driever (University of Essex)H-Index: 11
#2Andrew J. Simkin (University of Essex)H-Index: 24
Last. Christine A. Raines (University of Essex)H-Index: 40
view all 10 authors...
To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin–Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a Brachypodium distachyon SBPase gene con...
42 CitationsSource
#1Andrew J. Simkin (University of Essex)H-Index: 24
#2Lorna McAusland (University of Essex)H-Index: 7
Last. Christine A. Raines (University of Essex)H-Index: 40
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In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of the cyt b6f complex. Interestingly, an increase in the levels of proteins in both the photosystem I (PSI) and PSII complexes also was seen in the Rieske FeS overexpression plants. Al...
17 CitationsSource
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