Match!
Andrew J. Simkin
University of Essex
51Publications
23H-index
2,299Citations
Publications 51
Newest
#1Fiona WilsonH-Index: 1
#2Kate HarrisonH-Index: 1
Last.Richard J. HarrisonH-Index: 12
view all 0 authors...
Background Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recently Fragaria. In order to inform application of the technology in Fragaria, we targeted the visible endogenous marker gene PDS (phytoene desaturase) in diploid Fragaria vesca ssp. vesca ‘Hawaii 4’ and octoploid F. × ananassa ‘Calypso’.
#1Andrew J. Simkin (University of Essex)H-Index: 23
#2Patricia E. Lopez-Calcagno (University of Essex)H-Index: 5
Last.Christine A. Raines (University of Essex)H-Index: 37
view all 3 authors...
A number of recent studies have provided strong support demonstrating that improving the photosynthetic processes through genetic engineering can provide an avenue to improve yield potential. The major focus of this review is on improvement of the Calvin–Benson cycle and electron transport. Consideration is also given to how altering regulatory process may provide an additional route to increase photosynthetic efficiency. Here we summarize some of the recent successes that have been observed thr...
#1Saqer AlotaibiH-Index: 2
#2Caroline A. SparksH-Index: 17
Last.Christine A. RainesH-Index: 37
view all 5 authors...
Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bis...
Last.Andrew J. SimkinH-Index: 23
view all 6 authors...
Gateway technology has been used to facilitate the generation of a large number of constructs for the modification of plants for research purposes. However, many of the currently available vectors only allow the integration of a single cDNA of interest into an expression clone. The ability to over-express multiple genes in combination is essential for the study of plant development where several transcripts have a role to play in one or more metabolic processes. The tools to carry out such studi...
#1Steven M. Driever (University of Essex)H-Index: 11
#2Andrew J. Simkin (University of Essex)H-Index: 23
Last.Christine A. Raines (University of Essex)H-Index: 37
view all 10 authors...
To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin–Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a Brachypodium distachyon SBPase gene con...
#1Andrew J. Simkin (University of Essex)H-Index: 23
#2Lorna McAusland (University of Essex)H-Index: 7
Last.Christine A. Raines (University of Essex)H-Index: 37
view all 4 authors...
In this study, we generated transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b 6 f (cyt b 6 f ) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b 6 (PetB), core proteins of the cyt b 6 f complex. Interestingly, an increase in the levels of proteins in both the photosystem I (PSI) and PSII complexes also was seen in the Rieske FeS overexpression ...
#1Steven M. DrieverH-Index: 11
#2Andrew J. SimkinH-Index: 23
Last.Christine A. RainesH-Index: 37
view all 10 authors...
To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin–Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a Brachypodium distachyon SBPase gene con...
#1Andrew J. Simkin (University of Essex)H-Index: 23
#2Patricia E. Lopez-Calcagno (University of Essex)H-Index: 5
Last.Christine A. Raines (University of Essex)H-Index: 37
view all 8 authors...
© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. In this article, we have altered the levels of three different enzymes involved in the Calvin–Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7-bisphosphatase (SBPase), fructose 1,6-bisphophate aldolase (FBPA) and the glycine decarboxylase-H protein...
#1Andrew J. Simkin (University of Essex)H-Index: 23
#2Lorna McAusland (University of Nottingham)H-Index: 7
Last.Christine A. Raines (University of Essex)H-Index: 37
view all 4 authors...
In this study we have generated transgenic Arabidopsis plants over-expressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein, resulted in the concomitant increase in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of the cyt b6f complex. Interestingly, an increase in the levels of proteins in both the PSI and PSII complexes was also seen in the Rieske FeS ox plants. Although the mechanisms leading to these cha...
123456