Deya Cherpokova
Harvard University
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Publications 19
#1Patrick Münzer (Harvard University)
#2Roberto Negro (Harvard University)H-Index: 1
Last. Denisa D. Wagner (Harvard University)H-Index: 105
view all 13 authors...
Neutrophil extracellular trap formation (NETosis) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome assembly are associated with a similar spectrum of disorders. We hypothesized that NLRP3 inflammasome may participate in NETosis. Using confocal microscopy, we observed the formation of ASC speck in stimulated neutrophils in the absence of infection, as well as the association of ASC speck with the released NETs. Peptidylarginine deiminase (PAD) 4, a mediator of NETosis, was needed...
#1Deya Cherpokova (Harvard University)H-Index: 10
#2Charlotte C. Jouvene (Brigham and Women's Hospital)H-Index: 3
Last. Charles N. Serhan (Brigham and Women's Hospital)H-Index: 138
view all 9 authors...
Deep vein thrombosis (DVT) is a common cardiovascular disease with a major impact on quality of life, and safe and effective therapeutic measures to efficiently reduce existent thrombus burden are scarce. Using a comprehensive targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS)‑based metabololipidomics approach, we established temporal clusters of endogenously biosynthesized specialized pro-resolving mediators (SPMs) and proinflammatory and prothrombotic lipid mediators during DVT...
12 CitationsSource
#1Nicoletta Sorvillo (Harvard University)H-Index: 10
#2Daniella M. Mizurini (Harvard University)H-Index: 1
Last. Denisa D. Wagner (Harvard University)H-Index: 105
view all 14 authors...
Rationale: PAD4 (peptidylarginine deiminase type IV), an enzyme essential for neutrophil extracellular trap formation (NETosis), is released together with neutrophil extracellular traps into the ex...
4 CitationsSource
#1Nicoletta Sorvillo (Harvard University)H-Index: 10
#2Deya Cherpokova (Harvard University)H-Index: 10
Last. Denisa D. Wagner (Harvard University)H-Index: 105
view all 4 authors...
Neutrophils play a central role in innate immune defense. Advances in neutrophil biology have brought to light the capacity of neutrophils to release their decondensed chromatin and form large extr...
5 CitationsSource
#1Irina Pleines (University of Würzburg)H-Index: 19
#2Deya Cherpokova (Boston Children's Hospital)H-Index: 10
Last. Markus Bender (University of Würzburg)H-Index: 16
view all 3 authors...
AbstractMegakaryocytes differentiate from hematopoietic stem cells in the bone marrow. The transition of megakaryocytes to platelets is a complex process. Thereby, megakaryocytes extend proplatelets into sinusoidal blood vessels, where the proplatelets undergo fission to release platelets. Defects in platelet production can lead to a low platelet count (thrombocytopenia) with increased bleeding risk. Rho GTPases comprise a family of small signaling G proteins that have been shown to be master re...
3 CitationsSource
#1Ofir Wolach (TAU: Tel Aviv University)H-Index: 11
#2Rob S. Sellar (UCL: University College London)H-Index: 13
Last. Benjamin L. Ebert (MIT: Massachusetts Institute of Technology)H-Index: 74
view all 24 authors...
Thrombosis is a major cause of morbidity and mortality in Philadelphia chromosome–negative myeloproliferative neoplasms (MPNs), clonal disorders of hematopoiesis characterized by activated Janus kinase (JAK)–signal transducer and activator of transcription (STAT) signaling. Neutrophil extracellular trap (NET) formation, a component of innate immunity, has been linked to thrombosis. We demonstrate that neutrophils from patients with MPNs are primed for NET formation, an effect blunted by pharmaco...
39 CitationsSource
#1Shuchi Gupta (University of Würzburg)H-Index: 3
#2Deya Cherpokova (University of Würzburg)H-Index: 10
Last. Bernhard Nieswandt (University of Würzburg)H-Index: 64
view all 6 authors...
At sites of vascular injury, exposed subendothelial collagens trigger platelet activation and thrombus formation by interacting with the immunoreceptor tyrosine-based activation motif (ITAM)-coupled glycoprotein (GP) VI on the platelet surface. Platelets are derived from the cytoplasm of megakaryocytes (MKs), which extend large proplatelets into bone marrow (BM) sinusoids that are then released into the blood stream where the final platelet sizing and maturation occurs. The mechanisms that preve...
6 CitationsSource
#1Hideki HayashiH-Index: 21
#2Deya CherpokovaH-Index: 10
Last. Denisa D. WagnerH-Index: 105
view all 9 authors...
: Inflammation is a common denominator in chronic diseases of aging. Yet, how inflammation fuels these diseases remains unknown. Neutrophils are the primary leukocytes involved in the early phase of innate immunity and inflammation. As part of their anti-microbial defense, neutrophils form extracellular traps (NETs) by releasing decondensed chromatin lined with cytotoxic proteins. NETs have been shown to induce tissue injury and thrombosis. Here, we demonstrated that Sirt3, a nicotinamide adenin...
5 CitationsSource
#1Elizabeth J. Haining (University of Würzburg)H-Index: 2
#2Deya Cherpokova (University of Würzburg)H-Index: 10
Last. Bernhard Nieswandt (University of Würzburg)H-Index: 64
view all 9 authors...
C-type lectin-like receptor 2 (CLEC-2) is a platelet receptor that is critical during development in blood-lymph separation and implicated in thrombus stability in thrombosis and hemostasis. It is the only known platelet activatory receptor that participates in both of these aspects of platelet function, and it is the only one to signal through a hemi-immunoreceptor tyrosine–based activation motif (hemITAM). Current investigations into the function of CLEC-2 in vivo have focused on knockout (KO)...
13 CitationsSource
#1Sebastian Dütting (University of Würzburg)H-Index: 11
#2Frédérique Gaits-Iacovoni (French Institute of Health and Medical Research)H-Index: 19
Last. Bernhard Nieswandt (University of Würzburg)H-Index: 64
view all 26 authors...
Platelets derive from large precursor cells (megakaryocytes) in the bone marrow. Dutting et al . show that megakaryocyte polarization and platelet biogenesis in the bone-marrow sinusoids are directed by adhesion receptor GPIb signalling and resulting balanced antago…
15 CitationsSource