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Gerald Schatten
University of Pittsburgh
301Publications
71H-index
16.9kCitations
Publications 301
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#1Calvin Simerly (University of Pittsburgh)H-Index: 31
#2Diana Takahashi (Oregon National Primate Research Center)H-Index: 6
Last.Gerald Schatten (University of Pittsburgh)H-Index: 71
view all 8 authors...
With nearly ten million babies conceived globally, using assisted reproductive technologies, fundamental questions remain; e.g., How do the sperm and egg DNA unite? Does ICSI have consequences that IVF does not? Here, pronuclear and mitotic events in nonhuman primate zygotes leading to the establishment of polarity are investigated by multidimensional time-lapse video microscopy and immunocytochemistry. Multiplane videos after ICSI show atypical sperm head displacement beneath the oocyte cortex ...
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#1Joyce C. Harper (UCL: University College London)H-Index: 47
#2Gerald Schatten (University of Pittsburgh)H-Index: 71
Abstract Perhaps the two most significant pioneering biomedical discoveries with immediate clinical implications during the past forty years have been the advent of assisted reproductive technologies (ART) and the genetics revolution. ART, including in vitro fertilization (IVF), intracytoplasmic sperm injection and preimplantation genetic testing, has resulted in the birth of more than 8 million children, and the pioneer of IVF, Professor Bob Edwards, was awarded the 2010 Nobel Prize. The geneti...
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#1Calvin Simerly (University of Pittsburgh)H-Index: 31
#2Marion Manil-Ségalen (PSL Research University)H-Index: 2
Last.Gerald Schatten (University of Pittsburgh)H-Index: 71
view all 8 authors...
Oocytes, including from mammals, lack centrioles, but neither the mechanism by which mature eggs lose their centrioles nor the exact stage at which centrioles are destroyed during oogenesis is known. To answer questions raised by centriole disappearance during oogenesis, using a transgenic mouse expressing GFP-centrin-2 (GFP CETN2), we traced their presence from e11.5 primordial germ cells (PGCs) through oogenesis and their ultimate dissolution in mature oocytes. We show tightly coupled CETN2 do...
2 CitationsSource
#1Emily L. Fishman (UT: University of Toledo)H-Index: 3
#2Kyoung H. Jo (UT: University of Toledo)H-Index: 4
Last.Tomer Avidor-Reiss (UT: University of Toledo)H-Index: 23
view all 13 authors...
In the original version of this Article, the affiliation details for Jadranka Loncarek and Vito Mennella were incorrectly given as ‘Cell Biology Program, The Hospital for Sick Children, Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada’ and ‘Laboratory of Protein Dynamics and Signaling, Center for Cancer Research, National Cancer Institute, 1050 Boyles Street, Frederick, MD, 21702, USA’, respectively. This has now been corrected in both the PD...
3 CitationsSource
#1Emily L. Fishman (UT: University of Toledo)H-Index: 3
#2Kyoung H. Jo (UT: University of Toledo)H-Index: 4
Last.Tomer Avidor-Reiss (UT: University of Toledo)H-Index: 23
view all 13 authors...
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that the sperm centrosome contains, in addition to the known typical barrel-shaped centriole (the proximal centriole, PC), a surrounding matrix (pericentriolar material, PCM), and an atypical centriole (distal centriole, DC) composed of splayed microtubules surrounding previously undescribed rods of centriole luminal proteins. The sperm centrosome is remodeled by both reduction and enrichment of specifi...
19 CitationsSource
#1Gerald SchattenH-Index: 71
#2Calvin SimerlyH-Index: 31
Last.Jadranka Loncarek (NIH: National Institutes of Health)H-Index: 11
view all 8 authors...
Mammalian oocytes lack centrioles, but how or at what stage mature eggs lose their centrioles during oogenesis is unknown. To address centriole disappearance during oogenesis, using a transgenic mouse expressing GFP-centrin-2 (GFP CETN2), we traced their presence from e11.5 primordial germ cells (PGCs) through oogenesis and their ultimate dissolution in mature oocytes. We show tightly coupled CETN2 doublets in PGCs, oogonia, and pre-pubertal oocytes. Beginning with follicular recruitment of inco...
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#1Calvin SimerlyH-Index: 31
#2Carlos A. CastroH-Index: 16
Last.Gerald SchattenH-Index: 71
view all 7 authors...
Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm’s Release at Ejaculation
7 CitationsSource
#1Gerald Schatten (University of Pittsburgh)H-Index: 71
#2Tim Stearns (Stanford University)H-Index: 57
Summary Centrosomes are reduced to their cores in sperm. Emerging molecular explanations for centrosome construction have now helped to elucidate the mechanism of their destruction in sperm. Since centrosome inaccuracies cause aneuploidies responsible for cancers, birth defects and infertility, this new insight into centrosome behavior has broad implications.
1 CitationsSource
#1Gerald Schatten (University of Pittsburgh)H-Index: 71
#2Calvin Simerly (University of Pittsburgh)H-Index: 31
Centriole construction, now revealed by crystallography, proteomics, and imaging to be a sophisticated assembly of interlocking bricks, resembles LEGOs—albeit centrioles have remarkable dynamic capabilities, including self‐assembly and dis‐assembly, kinases and post‐translational modifications, self‐replication, and still mysterious mechanisms for transmission through each cell cycle and via the gametes during development. Centrioles are created by core proteins that aggregate to form unique nin...
4 CitationsSource
#1Gerald SchattenH-Index: 71
#2Tim StearnsH-Index: 57
Centrosomes are reduced to their cores in sperm. Emerging molecular explanations for centrosomeconstruction have now helped to elucidate the mechanism of their destruction in sperm. Sincecentrosome inaccuracies cause aneuploidies responsible for cancers, birth defects and infertility, this newinsight into centrosome behavior has broad implications.
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