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Laurens Liesenborghs
Katholieke Universiteit Leuven
Von Willebrand factorStaphylokinaseStaphylococcus aureusMedicineMicrobiology
25Publications
11H-index
887Citations
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Publications 25
Newest
#1Marijke Peetermans (Katholieke Universiteit Leuven)H-Index: 13
#2Severien Meyers (Katholieke Universiteit Leuven)H-Index: 1
Last. Peter Verhamme (Katholieke Universiteit Leuven)H-Index: 43
view all 12 authors...
BACKGROUND: Previous clinical evidence correlates levels of von Willebrand factor (VWF) and its cleaving protease ADAMTS13 with outcome in septic patients. No previous studies addressed if VWF and ADAMTS13 affected the outcome of Staphylococcus aureus sepsis. OBJECTIVES: We studied the role of VWF and ADAMTS13 in S. aureus sepsis both in patients and in mice. METHODS: VWF levels and ADAMTS13 activity levels were measured in plasma samples from 89 S. aureus bacteremia patients by chemiluminescent...
1 CitationsSource
#1Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
#2Severien Meyers (Katholieke Universiteit Leuven)H-Index: 1
Last. Peter Verhamme (Katholieke Universiteit Leuven)H-Index: 43
view all 4 authors...
Infective endocarditis is a life-threatening and enigmatic disease with a mortality of 30% and a pathophysiology that is poorly understood. However, at its core, an endocarditis lesion is mainly a fibrin and platelet blood clot infested with bacteria, clinging at the cardiac valves. Infective endocarditis therefore serves as a paradigm of immunothrombosis gone wrong. Immunothrombosis refers to the entanglement of the coagulation system with innate immunity and the role of coagulation in the isol...
Source
#1Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
#2Severien Meyers (Katholieke Universiteit Leuven)H-Index: 1
Last. Peter Verhamme (Katholieke Universiteit Leuven)H-Index: 43
view all 16 authors...
AIMS: The pathogenesis of endocarditis is not well understood resulting in unsuccessful attempts at prevention. Clinical observations suggest that Staphylococcus aureus infects either damaged or inflamed heart valves. Using a newly developed endocarditis mouse model, we therefore studied the initial adhesion of S. aureus in both risk states. METHODS AND RESULTS: Using 3D confocal microscopy, we examined the adhesion of fluorescent S. aureus to murine aortic valves. To mimic different risk states...
6 CitationsSource
#1Severien Meyers (Katholieke Universiteit Leuven)H-Index: 1
#2Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
Last. Kimberly Martinod (Katholieke Universiteit Leuven)H-Index: 22
view all 7 authors...
Source
#1Jorien Claes (Katholieke Universiteit Leuven)H-Index: 7
#2Bartosz Ditkowski (Katholieke Universiteit Leuven)H-Index: 3
Last. Ruth Heying (Katholieke Universiteit Leuven)H-Index: 16
view all 10 authors...
Adhesion of Staphylococcus aureus to endothelial cells (ECs) is paramount in infective endocarditis. Bacterial proteins such as clumping factor A (ClfA) and fibronectin binding protein A (FnbpA) mediate adhesion to EC surface molecules and (sub)endothelial matrix proteins including fibrinogen (Fg), fibrin, fibronectin (Fn) and von Willebrand factor (vWF). We studied the influence of shear flow and plasma on the binding of ClfA and FnbpA (including its sub-domains A, A 16+ , ABC, CD) to coverslip...
3 CitationsSource
#1Marijke Peetermans (Katholieke Universiteit Leuven)H-Index: 13
#2Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
Last. Thomas Vanassche (Katholieke Universiteit Leuven)H-Index: 19
view all 12 authors...
Background Staphylococcus aureus ( S. aureus ) bacteraemia is frequent and carries a high morbidity and mortality. Coagulases secreted by S. aureus initiate blood coagulation by directly activating prothrombin. This pathogen-activated coagulation is insensitive to most antithrombotic drugs, with the exception of small molecule direct thrombin inhibitors (DTIs). DTIs inhibit the coagulase-prothrombin complex, or staphylothrombin, and improve outcome in preclinical models of S. aureus infection. O...
Source
#1Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
#2Peter Verhamme (Katholieke Universiteit Leuven)H-Index: 43
Last. Thomas Vanassche (Katholieke Universiteit Leuven)H-Index: 19
view all 3 authors...
: The coagulation system does not only offer protection against bleeding, but also aids in our defense against invading microorganisms. The hemostatic system and innate immunity are strongly entangled, which explains why so many infections are complicated by either bleeding or thrombosis. Staphylococcus aureus (S. aureus), currently the most deadly infectious agent in the developed world, causes devastating intravascular infections such as sepsis and infective endocarditis. During these infectio...
7 CitationsSource
#1Jorien Claes (Katholieke Universiteit Leuven)H-Index: 7
#2Laurens Liesenborghs (Katholieke Universiteit Leuven)H-Index: 11
Last. Thomas Vanassche (Katholieke Universiteit Leuven)H-Index: 19
view all 12 authors...
Essentials Staphylococcus aureus (S. aureus) binds to endothelium via von Willebrand factor (VWF). Secreted VWF-binding protein (vWbp) mediates S. aureus adhesion to VWF under shear stress. vWbp interacts with VWF and the Sortase A-dependent surface protein Clumping factor A (ClfA). VWF-vWbp-ClfA anchor S. aureus to vascular endothelium under shear stress. Objective When establishing endovascular infections, Staphylococcus aureus (S. aureus) overcomes shear forces of flowing blood by binding to ...
19 CitationsSource
#1Marijke Peetermans (Katholieke Universiteit Leuven)H-Index: 13
#2Thomas Vanassche (Katholieke Universiteit Leuven)H-Index: 19
Last. Peter Verhamme (Katholieke Universiteit Leuven)H-Index: 43
view all 5 authors...
AbstractBoth coagulation and fibrinolysis are tightly connected with the innate immune system. Infection and inflammation cause profound alterations in the otherwise well-controlled balance between coagulation and fibrinolysis. Many pathogenic bacteria directly exploit the host’s hemostatic system to increase their virulence. Here, we review the capacity of bacteria to activate plasminogen. The resulting proteolytic activity allows them to breach tissue barriers and evade innate immune defense, ...
16 CitationsSource
#1Maarten Criel (Katholieke Universiteit Leuven)H-Index: 5
#2Benedetta Izzi (Katholieke Universiteit Leuven)H-Index: 18
Last. Marc Hoylaerts (Katholieke Universiteit Leuven)H-Index: 59
view all 11 authors...
Abstract Background Platelet Endothelial Aggregation Receptor-1 (PEAR1) is a transmembrane platelet receptor that amplifies the activation of the platelet fibrinogen receptor (αIIbβ3) during platelet aggregation. In man, Pear1 polymorphisms are associated with changes in platelet aggregability. In this report, we characterized Pear1 expression and function in murine platelets. Methods Pear1 phosphorylation and signaling, platelet aggregation, α-degranulation and clot retraction were studied in W...
3 CitationsSource
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