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Ian Slaymaker
Massachusetts Institute of Technology
31Publications
10H-index
4,009Citations
Publications 31
Newest
#1Ian SlaymakerH-Index: 10
#2Pablo Mesa (UCPH: University of Copenhagen)H-Index: 8
Last.Feng ZhangH-Index: 115
view all 13 authors...
Summary Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members. Cas13b, which has been used for both RNA editing and nucleic acid detection, is unique among type VI CRISPR effectors in its linear domain architecture and CRISPR RNA (crRNA) structure. Here, we report the crystal structure of Prevotella buccae Cas13b (PbuCas13b) bound to crRNA at 1.65 A resolution. This structure, combined with biochemical experiment...
#1Narin S. Tangprasertchai (SC: University of Southern California)H-Index: 3
#2Rosa Di Felice (SC: University of Southern California)H-Index: 26
Last.Peter Z. Qin (SC: University of Southern California)H-Index: 23
view all 8 authors...
The RNA-guided CRISPR–Cas9 nuclease has revolutionized genome engineering, yet its mechanism for DNA target selection is not fully understood. A crucial step in Cas9 target recognition involves unwinding of the DNA duplex to form a three-stranded R-loop structure. Work reported here demonstrates direct detection of Cas9-mediated DNA unwinding by a combination of site-directed spin labeling and molecular dynamics simulations. The results support a model in which the unwound nontarget strand is st...
#2Yang Zhou (McGovern Institute for Brain Research)H-Index: 8
Last.Feng ZhangH-Index: 115
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Summary Autism spectrum disorder (ASD) is a heterogeneous disease, but genetically defined models can provide an entry point to studying the molecular underpinnings of this disorder. We generated germline mutant mice with loss-of-function mutations in Chd8 , a de novo mutation strongly associated with ASD, and demonstrate that these mice display hallmark ASD behaviors, macrocephaly, and craniofacial abnormalities similar to patient phenotypes. Chd8 +/– mice display a broad, brain-region-specific...
Summary CRISPR-Cas adaptive immune systems defend microbes against foreign nucleic acids via RNA-guided endonucleases. Using a computational sequence database mining approach, we identify two class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, Csx27 and Csx28. We establish that these CRISPR-Cas systems can achieve RNA interference when heterologously expresse...
Last.Osamu NurekiH-Index: 56
view all 11 authors...
L'invention concerne des systemes, des procedes et des compositions pour le ciblage d'acides nucleiques. En particulier, l'invention concerne des systemes de ciblage d'ADN ou d'ARN non naturels ou modifies comprenant une nouvelle proteine effectrice CRISPR pour le ciblage de l'ADN ou de l'ARN et au moins un constituant du type acide nucleique de ciblage tel qu'un ARN guide.
#1Omar O. AbudayyehH-Index: 19
Last.Feng ZhangH-Index: 115
view all 15 authors...
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive immune systems, which protect microbes against viruses and other foreign DNA. All functionally characterized CRISPR systems have been reported to target DNA, with some multicomponent type III systems also targeting RNA. The putative class 2 type VI system, which has not been functionally characterized, encompasses the single-e...
#1Feng ZhangH-Index: 1
#2Linyi GaoH-Index: 6
Last.Ian SlaymakerH-Index: 10
view all 4 authors...
L'invention concerne une ou plusieurs mutations ou une ou plusieurs modifications de l'enzyme CRISPR, comme par exemple une enzyme Cas telle qu'une Cas9, permettant d'ameliorer, par exemple de diminuer, les effets non cibles d'un systeme ou d'un complexe CRISPR-Cas ou CRISPR-enzyme ou CRISPR-Cas9 qui contient ou qui comprend une enzyme Cas ou CRISPR ou une Cas9 mutee ou modifiee de ce type. L'invention concerne egalement des procedes de fabrication et d'utilisation associes, ainsi que des utilis...
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