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Douglas V. Sumerford
Iowa State University
31Publications
17H-index
725Citations
Publications 31
Newest
#1Blair D. Siegfried (NU: University of Nebraska–Lincoln)H-Index: 42
#2Murugesan Rangasamy (NU: University of Nebraska–Lincoln)H-Index: 12
Last.Nicholas P. Storer (Dow AgroSciences)H-Index: 21
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BACKGROUND: Transgenic corn hybrids that express toxins from Bacillus thuringiensis (Bt) have suppressed European corn borer populations and reduced the pest status of this insect throughout much of the US corn belt. A major assumption of the high-dose/refuge strategy proposed for insect resistance management and Bt corn is that the frequency of resistance alleles is low so that resistant pests surviving exposure to Bt corn will be rare. RESULTS:ThefrequencyofresistancetotheCry1FBttoxinwasestima...
30 CitationsSource
#1Brad S. Coates (Iowa State University)H-Index: 23
#2Douglas V. Sumerford (Iowa State University)H-Index: 17
Last.Craig A. Abel (Iowa State University)H-Index: 26
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Transgenic expression of Bacillus thuringiensis (Bt) crystalline (Cry) toxins by crop plants result in reduced insect feeding damage, but sustainability is threatened by the development of resistance traits in target insect populations. We investigated Bt toxin resistance trait in a laboratory colony of the European corn borer, Ostrinia nubilalis, selected for increased survival when exposed to Cry1Ab and correlated survival on Cry1Ab toxin with a constitutive w146.2 � 17.3-fold reduction in mid...
23 CitationsSource
#1Brad S. Coates (Iowa State University)H-Index: 23
#2Douglas V. Sumerford (Iowa State University)H-Index: 17
Last.Blair D. Siegfried (NU: University of Nebraska–Lincoln)H-Index: 42
view all 11 authors...
The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae...
17 CitationsSource
#1Brad S. Coates (ARS: Agricultural Research Service)H-Index: 23
#2J. A. Kroemer (ARS: Agricultural Research Service)H-Index: 1
Last.Richard L. Hellmich (Iowa State University)H-Index: 28
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The movement of miniature inverted repeat transposable elements (MITEs) modifies genome structure and function. We describe the microsatellite-associated interspersed nuclear element 2 (MINE-2), that integrates at consensus WTTTT target sites, creates dinucleotide TT target site duplications (TSDs), and forms predicted MITE-like secondary structures; a 5′ subterminal inverted repeat (SIR; AGGGTTCCGTAG) that is partially complementary to a 5′ inverted repeat (IR; ACGAAGCCCT) and 3′-SIRs (TTACGGAA...
22 CitationsSource
#1Miriam D. Lopez (ARS: Agricultural Research Service)H-Index: 6
#2Douglas V. Sumerford (Iowa State University)H-Index: 17
Last.Leslie C. Lewis (Iowa State University)H-Index: 31
view all 3 authors...
Abstract Infection with Nosema pyrausta Paillot lengthens developmental period of Bt-susceptible Ostrinia nubilalis (Hubner) to a similar extent as feeding on Cry1Ab-incorporated diet in Cry1Ab-resistant O. nubilalis, and these two factors combined lengthen developmental period further than either alone. Resistant O. nubilalis mating with infected susceptible, or infected resistant partners would produce partially- and fully-resistant offspring, respectively, infected with N. pyrausta. To invest...
5 CitationsSource
#1Douglas V. Sumerford (Iowa State University)H-Index: 17
#2John Glasser (EPA: United States Environmental Protection Agency)H-Index: 1
Last.L. C. Lewis (Iowa State University)H-Index: 4
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Many bioassays of insect species are dependent on the use of laboratory-reared insects. If the purpose of the research is to assess the genetic variance present for an insect trait, e.g., insecticide-resistance monitoring, it is imperative to understand the potential mating dynamics and genetic contributions of adults to the larvae evaluated in bioassays. We report the results of a study utilizing a laboratory-reared colony of Ostrinia nubilalis (Hubner). The changes in the population dynamics (...
1 CitationsSource
#1Faruque U. Zaman (PSU: Pennsylvania State University)
#2Dennis D. Calvin (PSU: Pennsylvania State University)H-Index: 17
Last.Douglas V. Sumerford (Iowa State University)H-Index: 17
view all 4 authors...
Synchronization between a parasitoid and its preferred host is an essential strategy for successful biological control. Two ecotypes of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) in North America are distinguished by their voltinism. In this study, the differential impact of a specialist parasitoid, Macrocentrus cingulum Brischke (Hymenoptera: Braconidae), on the univoltine and multivoltine populations of O. nubilalis is investigated. Four years of field and laboratory study suggested ...
Source
#1Brad S. Coates (Iowa State University)H-Index: 23
#2Douglas V. Sumerford (Iowa State University)H-Index: 17
Last.Leslie C. Lewis (Iowa State University)H-Index: 31
view all 4 authors...
Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A + T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA)n microsatellite loci, and was named the...
33 CitationsSource
#1Miriam D. Lopez (ARS: Agricultural Research Service)H-Index: 6
#2Douglas V. Sumerford (Iowa State University)H-Index: 17
Last.Leslie C. Lewis (Iowa State University)H-Index: 31
view all 3 authors...
The high dose/refixge strategy for delaying evolution of resistance to Bt maize [Zea mays L. (Poaceae) ] relies on random mating between resistant European corn borers, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae), and susceptible O. nubilalis from the refuge. However, differences in developmental rate caused by feeding on Bt maize, or infection with the microsporidium Nosema pyrausta Paillot (Microsporida: Nosematidae) may result in assortative mating. Developmental delays and mortality...
14 CitationsSource
#1Brad S. CoatesH-Index: 23
Last.Leslie C. LewisH-Index: 31
view all 7 authors...
Microsatellite loci are standard genetic markers for population genetic analysis, whereas single nucleotide polymorphisms (SNPs) are more recent tools that require assessment of neutrality and appropriate use in population genetics. Twelve SNP markers were used to describe the genetic structure of Diabrotica virgifera virgifera (LeConte; Coleoptera: Chrysomelidae) in the United States of America and revealed a high mean observed heterozygosity (0.40 ± 0.059) and low global FST (0.029). Pairwise ...
79 CitationsSource
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