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Ville Pihlajaniemi
VTT Technical Research Centre of Finland
26Publications
8H-index
216Citations
Publications 26
Newest
#1Marketta RinneH-Index: 22
#2Erika WinquistH-Index: 1
Last.Matti Siika-aho (VTT Technical Research Centre of Finland)H-Index: 40
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Abstract Grass is a versatile raw material for green biorefineries and preserving it as silage provides a year-round feedstock. The objective of the current study was to evaluate the effect of fibrolytic enzyme application on silage as a feedstock for a biorefinery. Two batches of grass (mixture of timothy and meadow fescue) silages were ensiled in pilot scale after fibrolytic enzyme was applied to them at four levels. Enzyme application increased fibre degradation linearly during ensiling and i...
Source
#1Ville Pihlajaniemi (VTT Technical Research Centre of Finland)H-Index: 8
#2Simo Ellilä (VTT Technical Research Centre of Finland)H-Index: 2
Last.Matti Siika-aho (VTT Technical Research Centre of Finland)H-Index: 40
view all 7 authors...
Abstract Grass silage is a promising biorefinery feedstock with surplus production potential, providing a source of readily soluble protein and lignocellulosic fibre. This study presents a concept combining protein extraction with production of single cell protein from enzymatically saccharified grass silage fibre by fermentation of the filamentous fungus Paecilomyces variotii. Steam explosion and ammonia soaking were compared as pretreatments, leading to 81.2% and 88.1% carbohydrate hydrolysabi...
Source
#1Riin Kont (UT: University of Tartu)H-Index: 4
#2Ville Pihlajaniemi (VTT Technical Research Centre of Finland)H-Index: 8
Last.Priit Väljamäe (UT: University of Tartu)H-Index: 19
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Background Enzyme-aided valorization of lignocellulose represents a green and sustainable alternative to the traditional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are important components of the state-of-the art enzyme cocktails for cellulose conversion. Yet, these monocopper enzymes are poorly characterized in terms of their kinetics, as exemplified by the growing evidence for that H2O2 may be a more efficient co-substrate for LPMOs than O2. LPMOs ne...
Source
#1Demi T. Djajadi (DTU: Technical University of Denmark)H-Index: 3
#2Ville PihlajaniemiH-Index: 8
Last.Anne S. Meyer (DTU: Technical University of Denmark)H-Index: 61
view all 5 authors...
1 CitationsSource
1 CitationsSource
#1Ossi Pastinen (Aalto University)H-Index: 15
#2Antti Nyyssölä (Aalto University)H-Index: 12
Last.Mika Henrikki Sipponen (Aalto University)H-Index: 11
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Abstract A new process is described for the two phase extraction of ergosterol and trehalose from microbial biomass. Baker’s yeast was used as a model organism to develop the method, which was then applied for extracting 13 oleaginous microbes. Major findings of the study were that the ergosterol content was not dependent on intracellular oil content and that 1-butanol and alkaline pH were needed to protect ergosterol. Saponification for 3–4 h at 85–100 °C followed by extraction of the reaction ...
4 CitationsSource
#1Piritta Niemi (VTT Technical Research Centre of Finland)H-Index: 7
#2Ville Pihlajaniemi (VTT Technical Research Centre of Finland)H-Index: 8
Last.Matti Siika-aho (VTT Technical Research Centre of Finland)H-Index: 40
view all 4 authors...
Abstract Grass is an underutilized potential feedstock for lignocellulosic sugar production for biorefinery applications and can be stabilized by ensiling for year-round supply. This study compared soaking in aqueous ammonia and steam explosion with dilute acid as pretreatments for enzymatic saccharification of grass silage. Both treatments led to high hydrolysability of the silage carbohydrates. An ammonia loading of 10% per DM was sufficient in an overnight soaking at 90 °C whereas the maximum...
7 CitationsSource
#1Mika Henrikki Sipponen (Aalto University)H-Index: 11
#2Orlando J. RojasH-Index: 50
Last.Monika Österberg (Aalto University)H-Index: 31
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Slow-release fertilizers represent a possible large-scale application for plant polymers. Here we show a facile way to stabilize urea in fertilizer systems by lignin. Chelation of kraft black liquor with calcium acetate at pH 13 precipitated lignin as a calcium complex (Ca-lignin), which offered beneficial effects if compared to those from lignin obtained by precipitation at low pH (Acid-lignin). The reduced affinity of water to Ca-lignin was exploited in the formulation of slow-release fertiliz...
7 CitationsSource
#1Marketta RinneH-Index: 22
#2Erika WinquistH-Index: 1
Last.Matti Siika-ahoH-Index: 40
view all 6 authors...
#1Marketta RinneH-Index: 22
#2Erika WinquistH-Index: 1
view all 7 authors...
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